Journal: Frontiers in Bioengineering and Biotechnology

Article Title: LIPUS-S/B@NPs regulates the release of SDF-1 and BMP-2 to promote stem cell recruitment-osteogenesis for periodontal bone regeneration

doi: 10.3389/fbioe.2023.1226426

Figure Lengend Snippet: Schematic diagram depicting the design principle of the biomimetic LIPUS assisted S/B@NPs-delivery system recruiting and facilitating the osteogenesis of stem cell in rat periodontal defect area under the radiation of LIPUS.

Article Snippet: Inspired by the natural bone healing cascade, a biomimetic LIPUS assisted drug delivery system was successfully designed and fabricated in this study, which could dually release SDF-1 and BMP-2 coupling the stem cell recruitment and osteogenesis processes.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: LIPUS-S/B@NPs regulates the release of SDF-1 and BMP-2 to promote stem cell recruitment-osteogenesis for periodontal bone regeneration

doi: 10.3389/fbioe.2023.1226426

Figure Lengend Snippet: Synthesis and characterization of S/B@NPs. (A) TEM image of S/B@NPs. (B) SEM image of S/B@NPs. (C) Particle size distributions of S/B@NPs. (D) Zeta potentials of S/B@NPs. (E) Release profiles of the SDF-1 from S/B@NPs with or without LIPUS treatment. (F) Release profiles of the BMP-2 from S/B@NPs with or without LIPUS treatment. Error bar represents the mean ± SD ( n = 3).

Article Snippet: Inspired by the natural bone healing cascade, a biomimetic LIPUS assisted drug delivery system was successfully designed and fabricated in this study, which could dually release SDF-1 and BMP-2 coupling the stem cell recruitment and osteogenesis processes.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: LIPUS-S/B@NPs regulates the release of SDF-1 and BMP-2 to promote stem cell recruitment-osteogenesis for periodontal bone regeneration

doi: 10.3389/fbioe.2023.1226426

Figure Lengend Snippet: Biocompatibility evaluation of LIPUS-S/B@NPs and its effect on cell migration. (A) CCK-8 results of S/B@NPs in day 1 and day 3 of culture. (B) Results of Live (green)/Dead (red) fluorescence assay of hPDLCs after 1 and 3 days of co-culture. Scale bar: 100 μm. (C) Optical images of hPDLCs recruited in the transwell assay and (D) their quantification. Error bar represents the mean ± SD ( n = 3); *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Inspired by the natural bone healing cascade, a biomimetic LIPUS assisted drug delivery system was successfully designed and fabricated in this study, which could dually release SDF-1 and BMP-2 coupling the stem cell recruitment and osteogenesis processes.

Techniques: Migration, CCK-8 Assay, Fluorescence, Co-Culture Assay, Transwell Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: LIPUS-S/B@NPs regulates the release of SDF-1 and BMP-2 to promote stem cell recruitment-osteogenesis for periodontal bone regeneration

doi: 10.3389/fbioe.2023.1226426

Figure Lengend Snippet: The effect of LIPUS-S/B@NPs on cell osteogenesis. (A) ALP staining and alizarin red staining images of hPDLCs. (B) Quantification of ARS staining, and gene expressions of (C) ALP , (D) Runx2 , (E) Col1a1 . Error bar represents the mean ± SD ( n = 3); *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Inspired by the natural bone healing cascade, a biomimetic LIPUS assisted drug delivery system was successfully designed and fabricated in this study, which could dually release SDF-1 and BMP-2 coupling the stem cell recruitment and osteogenesis processes.

Techniques: Staining

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: LIPUS-S/B@NPs regulates the release of SDF-1 and BMP-2 to promote stem cell recruitment-osteogenesis for periodontal bone regeneration

doi: 10.3389/fbioe.2023.1226426

Figure Lengend Snippet: The effect of LIPUS-S/B@NPs on cell osteogenesis. (A) Immunofluorescence images stained with Runx2 (green) and DAPI (blue) and (B) its quantitative assessment. Error bar represents the mean ± SD ( n = 3); *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Inspired by the natural bone healing cascade, a biomimetic LIPUS assisted drug delivery system was successfully designed and fabricated in this study, which could dually release SDF-1 and BMP-2 coupling the stem cell recruitment and osteogenesis processes.

Techniques: Immunofluorescence, Staining

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: LIPUS-S/B@NPs regulates the release of SDF-1 and BMP-2 to promote stem cell recruitment-osteogenesis for periodontal bone regeneration

doi: 10.3389/fbioe.2023.1226426

Figure Lengend Snippet: The effect of LIPUS-S/B@NPs on stem cell recruitment in vivo and its biocompatibility in vivo . (A) Schematic diagram illustrating the time frame of the in vivo study. (B) Confocal fluorescence images of hard tissue sections of periodontal bone defect area in rats to observe the recruited CFSE-labelled rBMSCs (green fluorescence) and (C) quantitative analysis of the fluorescence intensity. (D) H&E staining of heart, liver, spleen, lung, kidney tissues of SD rats after 8 weeks’ treatment. Scale bar: 100 μm. Error bar represents the mean ± SD ( n = 3); *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Inspired by the natural bone healing cascade, a biomimetic LIPUS assisted drug delivery system was successfully designed and fabricated in this study, which could dually release SDF-1 and BMP-2 coupling the stem cell recruitment and osteogenesis processes.

Techniques: In Vivo, Fluorescence, Staining

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: LIPUS-S/B@NPs regulates the release of SDF-1 and BMP-2 to promote stem cell recruitment-osteogenesis for periodontal bone regeneration

doi: 10.3389/fbioe.2023.1226426

Figure Lengend Snippet: LIPUS-S/B@NPs promoted periodontal regeneration in rats. (A) Sagittal, vertical and cross-section micro-CT scanning images of rat periodontal bone defects. The new bone in the defect area is marked in yellow and the HT particles in blue. Scale bar: 1 mm. (B) Quantitative analysis of bone microarchitectural parameters determined by micro-CT. Error bar represents the mean ± SD ( n = 3); *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Inspired by the natural bone healing cascade, a biomimetic LIPUS assisted drug delivery system was successfully designed and fabricated in this study, which could dually release SDF-1 and BMP-2 coupling the stem cell recruitment and osteogenesis processes.

Techniques: Micro-CT

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: LIPUS-S/B@NPs regulates the release of SDF-1 and BMP-2 to promote stem cell recruitment-osteogenesis for periodontal bone regeneration

doi: 10.3389/fbioe.2023.1226426

Figure Lengend Snippet: LIPUS-S/B@NPs promoted periodontal regeneration in rats. (A) H&E staining images of periodontal bone tissue sections. The defect area is marked with a green dotted line. (B) Masson-trichrome staining images of periodontal bone tissue sections. The defect area is marked with a red dotted line. Scale bar in black: 500 μm; scale bar in red: 50 μm. The yellow arrows refer to the implanted HT particles area.

Article Snippet: Inspired by the natural bone healing cascade, a biomimetic LIPUS assisted drug delivery system was successfully designed and fabricated in this study, which could dually release SDF-1 and BMP-2 coupling the stem cell recruitment and osteogenesis processes.

Techniques: Staining